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JCM 1048 . METHANOGEN HIGH SALT MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1048 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.40 g 22.457 384.271
MgCl2 x 6 H2O 11.00 g 10.557 51.926
NaHCO3 3.80 g 3.647 43.411
NH4Cl 1.00 g 0.96 17.942
KCl 1.00 g 0.96 12.873
KH2PO4 0.68 g 0.653 4.795
CaCl2 x 2 H2O 0.30 g 0.288 1.958
Wolfe's mineral solution 10.00 ml - -
Trace vitamins 10.00 ml - -
Resazurin 1.00 mg 9.6e-4 0.004
Sodium acetate 1.00 g 0.96 11.699
Yeast extract 2.00 g 1.919 -
Distilled water 1000.00 ml - -
1 Mix components, except NaHCO3, and adjust pH to 7.0. Bring to a boil for 5-10 sec, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the medium. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers and autoclave. After autoclaving, stand the medium overnight. Prior to use, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Methanol
(v/v, 50%) 		10.00 ml - -
L-Cysteine HCl x H2O
(5%) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		2.00 ml - -
2 Comment: For strain JCM 30226, supplement with 20 mM (final) tetramethylammonium as a substrate instead of methanol.

Wolfe's mineral solution #

1000
Solution occurs in 7 other media (first defined in Medium J265).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace minerals 1000.00 ml - -
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2SeO3 1.00 mg 1.0e-3 -
Na2WO4 x 2 H2O 0.01 g 0.01 0.03

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 22.466 384.435
MgCl2 x 6 H2O 10.557 51.926
NaHCO3 3.647 43.411
Yeast extract 1.919 -
NH4Cl 0.96 17.942
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains