J1057
1057 .
ACETOMICROBIUM FAECALE MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1057 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD-BBL) |
2.000 | g | 1.961 | - | |
| Yeast extract | 2.000 | g | 1.961 | - | |
| Glucose | 4.000 | g | 3.922 | 21.767 | |
| KH2PO4 | 0.225 | g | 0.221 | 1.621 | |
| K2HPO4 | 0.255 | g | 0.25 | 1.435 | |
| (NH4)2SO4 | 0.255 | g | 0.25 | 1.892 | |
| NaCl | 0.500 | g | 0.49 | 8.388 | |
| MgSO4 x 7 H2O | 0.100 | g | 0.098 | 0.398 | |
| CaCl2 x 2 H2O | 0.070 | g | 0.069 | 0.467 | |
| Sodium acetate x 3 H2O | 5.000 | g | 4.902 | - | |
| Trace vitamins | 10.000 | ml | - | - | |
| Trace minerals solution | 10.000 | ml | - | - | |
| Resazurin | 1.000 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix components thoroughly. Bring to a boil for seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture and add the following components to the medium: | |||||
| NaHCO3 | 6.000 | g | 5.882 | 70.022 | |
| L-Cysteine HCl x H2O | 0.500 | g | 0.49 | - | |
| 2 Seal with butyl rubber stoppers and autoclave. After inoculation, pressurize the inoculated bottles to 200 kPa with a N2-CO2 (4:1, v/v) gas mixture. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.00 | ml | - | - |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 |
| Na2SeO3 x 5 H2O | 0.30 | mg | 3.0e-4 | 0.001 |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 5.882 | 70.022 |
| Sodium acetate x 3 H2O | 4.902 | - |
| Glucose | 3.922 | 21.767 |
| Trypticase peptone | 1.961 | - |
| Yeast extract | 1.961 | - |
| Show more | ||
