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J1131. SALINISPIRA L21 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 60.0 g 58.252 996.748
2 6.0 g 5.825 28.653
3 1.5 g 1.456 19.534
4 1.0 g 0.971 6.835
5 1.0 g 0.971 18.15
6 0.4 g 0.388 2.642
7 0.4 g 0.388 2.23
8 Trace mineral solution 10.0 ml - -
9 1.0 g 0.971 -
10
(BD-BBL)
1.0 g 0.971 -
11 0.5 mg 4.9e-4 0.002
12 950.0 ml 920.676 51105.2
13
(10%)
10.0 ml - -
14 Trace vitamins
(*)
10.0 ml - -
15
(*, 8%)
30.0 ml 5.031 59.884
16
(5%)
10.0 ml - -
17
(5%)
10.0 ml - -
Instructions:
  1. Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized): #13-15
  2. Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere): #16-17
  3. Readjust pH to 7.3-7.5, if necessary.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 4 other media (first defined in medium J1084 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace minerals 1000.00 ml - -
2 0.03 g 0.03 -
3 0.30 mg 3.0e-4 -
4 0.40 mg 4.0e-4 -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 940.059 52181.1
NaCl 58.262 996.914
MgCl2 x 6H2O 5.825 28.653
NaHCO3 5.031 59.884
KCl 1.456 19.534
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