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J1162. NAS-02 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.2 g 0.19 1.44
2 3.0 g 2.854 20.975
3 Modified Allen's trace metal solution 1.0 ml - -
4 2.5 g 2.379 -
5 1000.0 ml 949.769 52720.1
6
(w/v, 5%)
10.0 ml - -
7
(w/v, 5%)
5.0 ml - -
8 Trace vitamins
(*)
10.0 ml - -
9
(w/v, 10%)
5.0 ml - -
10
(25 mM)
10.0 ml - -
11
(130 mM)
10.0 ml 18.364 144.879
Instructions:
  1. Mix components thoroughly and adjust pH to 5.2. Autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N2 atmosphere): #6-11
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium J1014 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.80 g 1.8 -
2 4.50 g 4.5 -
3 0.22 g 0.22 -
4 0.05 g 0.05 -
5 0.03 g 0.03 0.124
6 0.03 g 0.03 -
7 0.01 g 0.01 -
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 960.216 53300
FeCl2 18.364 144.879
KH2PO4 2.854 20.975
Na2S2O3 x 5H2O 2.379 -
(NH4)2SO4 0.19 1.44
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