MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1186 . MARINE METHANOBACTERIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1186 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.10 g 0.096 0.706
MgCl2 x 6 H2O 4.00 g 3.842 18.9
CaCl2 x 2 H2O 1.00 g 0.961 6.534
NH4Cl 0.50 g 0.48 8.979
NaCl 25.00 g 24.015 410.941
Yeast extract 0.10 g 0.096 -
Sodium formate 0.68 g 0.653 9.605
Sodium acetate 0.08 g 0.077 0.937
Trace vitamins solution 2.00 ml - -
Trace mineral solution 1.00 ml - -
Selenite-tungstate solution
(Medium No. 852) 		1.00 ml - -
Resazurin 1.00 mg 9.6e-4 0.004
Distilled water 1000.00 ml - -
1 Mix components and adjust pH to 7.5. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		25.00 ml - -
L-Cysteine HCl x H2O
(5%) 		6.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -
2 Pressurize the inoculated vessels to 100 kPa H2-CO2 (4:1, v/v)

Trace vitamins solution #

1000
Solution occurs in 9 other media (first defined in Medium J284).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 4.9 mg 0.005 0.02
Folic acid 8.8 mg 0.009 0.02
Pyridoxine hydrochloride 4.1 mg 0.004 -
Thiamine HCl 6.7 mg 0.007 -
Riboflavin 7.5 mg 0.008 0.02
Nicotinic acid 2.5 mg 0.003 0.02
DL-Calcium pantothenate 9.5 mg 0.01 0.02
Vitamin B12 27.1 mg 0.027 0.02
p-Aminobenzoic acid 2.7 mg 0.003 0.02
Lipoic acid 4.1 mg 0.004 0.02
Distilled water 1000.0 ml - -

Trace mineral solution #

1000
Solution occurs in 8 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.990 g 1.99 10.01
MnCl2 x 4 H2O 0.198 g 0.198 -
CoCl2 x 6 H2O 0.130 g 0.13 1.001
ZnCl2 0.136 g 0.136 0.998
CuCl2 x 2 H2O 1.700 mg 0.002 0.01
H3BO3 6.200 mg 0.006 0.1
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.013 g 0.013 0.1
AlCl3 0.013 g 0.013 0.097
Distilled water 1000.000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.015 410.941
MgCl2 x 6 H2O 3.842 18.9
CaCl2 x 2 H2O 0.961 6.534
Sodium formate 0.653 9.605
NH4Cl 0.48 8.979
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain