Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 120.0 | g | 117.073 | 2003.31 | |
KCl | 1.5 | g | 1.463 | 19.63 | |
MgCl2 x 6 H2O | 6.0 | g | 5.854 | 28.793 | |
CaCl2 x 2 H2O | 0.4 | g | 0.39 | 2.654 | |
NH4Cl | 1.0 | g | 0.976 | 18.239 | |
K2HPO4 | 0.3 | g | 0.293 | 1.68 | |
Trace minerals | 10.0 | ml | - | - | |
Trace vitamins | 10.0 | ml | - | - | |
Yeast extract
(Oxoid) |
2.0 | g | 1.951 | - | |
Trypticase peptone
(BD-BBL) |
2.0 | g | 1.951 | - | |
Resazurin | 0.5 | mg | 4.9e-4 | 0.002 | |
Distilled water | 920.0 | ml | - | - | |
1 Mix components thoroughly and autoclave the medium under a N2:CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (filter-sterilized): | |||||
NaHCO3
(8%) |
50.0 | ml | - | - | |
Coenzyme M
(1.4%) |
10.0 | ml | - | - | |
Trimethylamine-HCl
(1 M) |
20.0 | ml | - | - | |
2 Adjust pH to 7.0-7.2, if necessary. Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml medium in 60 ml-serum bottles) and seal with butyl rubber stoppers. Add per liter the following solution (autoclaved and stored under a N2 atmosphere). | |||||
Na2S x 9 H2O
(5%) |
5.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | 7.1 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 117.083 | 2003.48 |
MgCl2 x 6 H2O | 5.854 | 28.793 |
Yeast extract | 1.951 | - |
Trypticase peptone | 1.951 | - |
KCl | 1.463 | 19.63 |
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