Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 2.00 | g | 1.919 | 32.844 | |
NH4Cl | 0.25 | g | 0.24 | 4.485 | |
KCl | 0.25 | g | 0.24 | 3.218 | |
KH2PO4 | 0.25 | g | 0.24 | 1.763 | |
MgSO4 x 7 H2O | 0.50 | g | 0.48 | 1.947 | |
CaCl2 x 2 H2O | 0.10 | g | 0.096 | 0.653 | |
MOPS | 2.09 | g | 2.006 | 9.585 | |
Yeast extract | 0.50 | g | 0.48 | - | |
SL-9 mineral solution | 10.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.50 | g | 0.48 | - | |
Resazurin | 0.50 | mg | 4.8e-4 | 0.002 | |
Distilled water | 990.00 | ml | - | - | |
1 Mix components thoroughly and adjust pH 7.0. Dispense the medium into culture vessels under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks (filter-sterilized): | |||||
Fructose
(1 M) |
30.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
Vitamin B12 solution 0.05 g/L | 1.00 | ml | - | - | |
Coenzyme M
(0.01%) |
1.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 2.0 | g | 2 | 10.464 | |
MnSO4 x n H2O | 1.0 | g | 1 | - | |
Fe(NH4)2(SO4)2 x 6 H2O | 0.8 | g | 0.8 | - | |
CoCl2 x 6 H2O | 0.2 | g | 0.2 | 1.54 | |
ZnSO4 x 7 H2O | 1.0 | mg | 1.0e-3 | 0.003 | |
CuCl2 x 2 H2O | 20.0 | mg | 0.02 | 0.117 | |
NiCl2 x 6 H2O | 20.0 | mg | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 20.0 | mg | 0.02 | 0.083 | |
Na2SeO4 | 20.0 | mg | 0.02 | 0.106 | |
Na2WO4 x 2 H2O | 20.0 | mg | 0.02 | 0.061 | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 5 | mg | 0.05 | 0.037 |
Distilled water | 100 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.5 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MOPS | 2.006 | 9.585 |
NaCl | 1.919 | 32.844 |
MgSO4 x 7 H2O | 0.48 | 1.947 |
L-Cysteine HCl x H2O | 0.48 | - |
Yeast extract | 0.48 | - |
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