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J1290. NRmbB1 MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.54 g 0.575 10.751
2 0.14 g 0.149 1.096
3 3.00 g 3.195 15.715
4 0.15 g 0.16 1.087
5 20.00 g 21.299 364.448
6 Trace mineral solution 1.00 ml - -
7 Se/W solution 1.00 ml - -
8 2.50 g 2.662 31.693
9 1.00 mg 0.001 0.005
10 900.00 ml 956.748 53107.5
11
(see Medium No. 197, *)
1.00 ml - -
12 Vitamin B12 solution
(*)
0.10 ml - -
13
(1.0 M)
20.00 ml - -
14
(0.5 M)
10.00 ml - -
15
(5%)
6.00 ml - -
Instructions:
  1. Mix components except NaHCO3 and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): #11-15
Solution occurs in 8 other media (first defined in medium J852 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.990 g 1.99 -
2 0.198 g 0.198 -
3 0.130 g 0.13 -
4 0.136 g 0.136 0.998
5 1.700 mg 0.002 -
6 6.200 mg 0.006 0.1
7 0.024 g 0.024 0.099
8 0.013 g 0.013 -
9 0.013 g 0.013 0.097
10 1000.000 ml 998.207 55408.8
Solution occurs in 3 other media (first defined in medium J852 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.7 mg 0.002 -
2 3.3 mg 0.003 -
3 1000.0 ml 998.207 55408.8
Solution occurs in 18 other media (first defined in medium J403 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 mg 0.05 0.032
2 100 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 958.98 53231.4
NaCl 21.299 364.448
MgCl2 x 6H2O 3.195 15.715
NaHCO3 2.662 31.693
NH4Cl 0.575 10.751
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