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JCM 1290 . NRmbB1 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1290 #

ml
Equipment needed: Anaerobic workbench Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.54 g 0.575 10.751
KH2PO4 0.14 g 0.149 1.096
MgCl2 x 6 H2O 3.00 g 3.195 15.715
CaCl2 x 2 H2O 0.15 g 0.16 1.087
NaCl 20.00 g 21.299 364.464
Trace mineral solution 1.00 ml - -
Se/W solution 1.00 ml - -
NaHCO3 2.50 g 2.662 31.693
Resazurin 1.00 mg 0.001 0.005
Distilled water 900.00 ml - -
1 Mix components except NaHCO3 and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
Vitamin solution
(see Medium No. 197, *) 		1.00 ml - -
Vitamin B12 solution 0.05 g/L
(*) 		0.10 ml - -
Glucose
(1.0 M) 		20.00 ml - -
Coenzyme M
(0.5 M) 		10.00 ml - -
Na2S x 9 H2O
(5%) 		6.00 ml - -

Trace mineral solution #

1000
Solution occurs in 8 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl2 x 4 H2O 1.990 g 1.99 10.01
MnCl2 x 4 H2O 0.198 g 0.198 -
CoCl2 x 6 H2O 0.130 g 0.13 1.001
ZnCl2 0.136 g 0.136 0.998
CuCl2 x 2 H2O 1.700 mg 0.002 0.01
H3BO3 6.200 mg 0.006 0.1
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
NiCl2 x 6 H2O 0.013 g 0.013 0.1
AlCl3 0.013 g 0.013 0.097
Distilled water 1000.000 ml - -

Se/W solution #

1000
Solution occurs in 3 other media (first defined in Medium J852).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 1.7 mg 0.002 0.006
Na2WO4 x 2 H2O 3.3 mg 0.003 0.01
Distilled water 1000.0 ml - -

Vitamin B12 solution 0.05 g/L #

100
Solution occurs in 18 other media (first defined in Medium J403).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 5 mg 0.05 0.037
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 21.299 364.464
MgCl2 x 6 H2O 3.195 15.715
NaHCO3 2.662 31.693
NH4Cl 0.575 10.751
CaCl2 x 2 H2O 0.16 1.087
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain