Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 0.54 | g | 0.575 | 10.751 | |
KH2PO4 | 0.14 | g | 0.149 | 1.096 | |
MgCl2 x 6 H2O | 3.00 | g | 3.195 | 15.715 | |
CaCl2 x 2 H2O | 0.15 | g | 0.16 | 1.087 | |
NaCl | 20.00 | g | 21.299 | 364.464 | |
Trace mineral solution | 1.00 | ml | - | - | |
Se/W solution | 1.00 | ml | - | - | |
NaHCO3 | 2.50 | g | 2.662 | 31.693 | |
Resazurin | 1.00 | mg | 0.001 | 0.005 | |
Distilled water | 900.00 | ml | - | - | |
1 Mix components except NaHCO3 and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): | |||||
Vitamin solution
(see Medium No. 197, *) |
1.00 | ml | - | - | |
Vitamin B12 solution 0.05 g/L
(*) |
0.10 | ml | - | - | |
Glucose
(1.0 M) |
20.00 | ml | - | - | |
Coenzyme M
(0.5 M) |
10.00 | ml | - | - | |
Na2S x 9 H2O
(5%) |
6.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
FeCl2 x 4 H2O | 1.990 | g | 1.99 | 10.01 |
MnCl2 x 4 H2O | 0.198 | g | 0.198 | - |
CoCl2 x 6 H2O | 0.130 | g | 0.13 | 1.001 |
ZnCl2 | 0.136 | g | 0.136 | 0.998 |
CuCl2 x 2 H2O | 1.700 | mg | 0.002 | 0.01 |
H3BO3 | 6.200 | mg | 0.006 | 0.1 |
Na2MoO4 x 2 H2O | 0.024 | g | 0.024 | 0.099 |
NiCl2 x 6 H2O | 0.013 | g | 0.013 | 0.1 |
AlCl3 | 0.013 | g | 0.013 | 0.097 |
Distilled water | 1000.000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Na2SeO3 x 5 H2O | 1.7 | mg | 0.002 | 0.006 |
Na2WO4 x 2 H2O | 3.3 | mg | 0.003 | 0.01 |
Distilled water | 1000.0 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Vitamin B12 | 5 | mg | 0.05 | 0.037 |
Distilled water | 100 | ml | - | - |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.5 |
Equipment needed: | Anaerobic workbench, Autoclave, Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 21.299 | 364.464 |
MgCl2 x 6 H2O | 3.195 | 15.715 |
NaHCO3 | 2.662 | 31.693 |
NH4Cl | 0.575 | 10.751 |
CaCl2 x 2 H2O | 0.16 | 1.087 |
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