J1298
1298 .
ABSS2
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1298 #
ml
Equipment needed: Filter
Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 11.8 | g | 11.535 | 197.377 | |
| MgCl2 x 6 H2O | 2.2 | g | 2.151 | 10.578 | |
| CaCl2 x 2 H2O | 0.3 | g | 0.293 | 1.995 | |
| KCl | 0.4 | g | 0.391 | 5.245 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 1 M) |
3.0 | ml | - | - | |
|
NH4Cl
(100 mM) |
20.0 | �l | - | - | |
|
Na2HPO4
(10 mM) |
100.0 | �l | - | - | |
| SL-7 trace element solution | 100.0 | �l | - | - | |
|
Vitamin solution
(*) |
420.0 | �l | - | - | |
|
Na2S2O3
(300 mM) |
10.0 | ml | - | - | |
|
MnO2
(500 mM) |
10.0 | ml | - | - | |
| 2 Adjust pH to 7.5 by bubbling with a N2-CO2 (4:1, v/v) gas mixture. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Vitamin B12 | 10 | mg | 0.01 | 0.007 |
| Inositol | 10 | mg | 0.01 | 0.056 |
| Biotin | 10 | mg | 0.01 | 0.041 |
| Folic acid | 10 | mg | 0.01 | 0.023 |
| p-Aminobenzoic acid | 100 | mg | 0.1 | 0.729 |
| Nicotinic acid | 1 | g | 1 | 8.123 |
| DL-Calcium pantothenate | 1 | g | 1 | 2.099 |
| Thiamine HCl | 2 | g | 2 | - |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium J536).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(7.7 M, 25%) |
10.0 | ml | - | - | |
| FeCl2 x 4 H2O | 1.5 | g | 1.485 | 7.47 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.188 | 1.449 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.099 | - | |
| ZnCl2 | 70.0 | mg | 0.069 | 0.508 | |
| H3BO3 | 62.0 | mg | 0.061 | 0.993 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.147 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.183 | |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.099 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Initially, dissolve FeCl2 x 4H2O in 25% HCl, then add distilled water followed by the other components. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 7.5 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |