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J14. EG MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Oxoid)
2.4 g 2.4 -
2
(BD-Difco)
10.0 g 10 -
3
(BD-Difco)
5.0 g 5 -
4 4.0 g 4 28.177
5 1.5 g 1.5 8.326
6 0.5 g 0.5 -
7 0.2 g 0.2 0.832
8 15.0 g 15 -
9 0.5 g 0.5 2.847
10 50.0 ml - -
11 950.0 ml 948.297 52638.4
Instructions:
  1. Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 953

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 948.297 52638.4
Agar 15 -
Proteose peptone No. 3 10 -
Yeast extract 5 -
Na2HPO4 4 28.177
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