MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 216 . JCM MEDIUM No. 216

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J216 #

ml
Equipment needed: Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.25 g 0.25 4.674
K2HPO4 0.05 g 0.05 0.287
KH2PO4 0.09 g 0.09 0.661
NaCl 11.80 g 11.8 201.916
MgSO4 x 7 H2O 1.75 g 1.75 7.1
MgCl2 x 6 H2O 4.50 g 4.5 22.134
Na2SO4 0.81 g 0.81 5.703
CaCl2 x 2 H2O 0.78 g 0.78 5.306
KCl 0.30 g 0.3 4.024
NaHCO3 1.00 g 1 11.904
Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg 0.002 -
(NH4)2Ni(SO4)2 x 6 H2O 2.00 mg 0.002 0.005
Na2WO4 x 2 H2O 2.00 mg 0.002 0.006
Trace minerals 10.00 ml - -
Marine trace elements solution 10.00 ml - -
Trace vitamins 10.00 ml - -
Resazurin 1.00 mg 1.0e-3 0.004
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 970.00 ml - -
1 Mix ingredients, except Na2S x 9H2O, adjust pH to 6.0 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Autoclave Na2S x 9H2O as a 5% solution under a N2 atmosphere. Prior to inoculation, anaerobically add the sterile Na2S x 9H2O solution, and readjust pH to 6.5 with sterile H2SO4. Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20).

Marine trace elements solution #

1000
Solution occurs in 1 other media (first defined in Medium J216).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaBr 4.00 g 4 38.877
SrCl2 x 6 H2O 1.80 g 1.8 6.751
H3BO3 1.30 g 1.3 21.022
KI 1.25 mg 0.001 0.005
Sodium silicate 100.00 mg 0.1 0.819
NaF 60.00 mg 0.06 1.429
KNO3 40.00 mg 0.04 0.396
Na2HPO4 x 12 H2O 0.46 g 0.46 3.24
Distilled water 1000.00 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 11.81 202.087
MgCl2 x 6 H2O 4.5 22.134
MgSO4 x 7 H2O 1.78 7.222
NaHCO3 1 11.904
Na2SO4 0.81 5.703
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain