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J232. METHANOCALDOCOCCUS JANNASCHII MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.14 g 0.14 0.804
2 0.14 g 0.14 0.952
3 0.25 g 0.25 4.674
4 3.40 g 3.4 13.794
5 2.70 g 2.7 13.281
6 0.33 g 0.33 4.427
7 0.75 mg 7.5e-4 -
8 0.50 mg 5.0e-4 -
9 30.00 g 30 513.325
10 0.01 g 0.01 -
11 Trace minerals 10.00 ml - -
12 Trace vitamins 10.00 ml - -
13 1.00 g 1 11.904
14 0.50 g 0.5 2.847
15 0.50 g 0.5 2.082
16 1.00 mg 1.0e-3 0.004
17 980.00 ml 978.243 54300.7
Instructions:
  1. Mix ingredients, except NaHCO3, cysteine x HCl and Na2S x 9H2O, and adjust pH to 6.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20).
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 8

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaCl 30.01 513.496
MgSO4 x 7H2O 3.43 13.916
MgCl2 x 6H2O 2.7 13.281
NaHCO3 1 11.904
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