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JCM 239 . JCM MEDIUM No. 239

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J239 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Asparagine 1 g 90.909 688.08
Glycerol 2 g 181.818 1974.35
K2HPO4 1 g 90.909 521.938
Polypeptone
(Nihon Pharm. Co.) 		30 g 2727.27 -
Trace salt solution 1 ml - -
Vitamin B solution 10 ml - -
Agar 15 g 1363.64 4054.46
1 Add components, except vitamin B solution, to distilled water and bring volume to 990 ml. Adjust pH to 7.2. After autoclaving, aseptically add filter-sterilized vitamin B solution.

Trace salt solution #

100
Solution occurs in 6 other media (first defined in Medium 547).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 5.053
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -

Vitamin B solution #

200
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.05 -
Riboflavin 10 mg 0.05 0.133
Nicotinate 10 mg 0.05 0.41
Pyridoxine hydrochloride 10 mg 0.05 -
Myo-inositol 10 mg 0.05 0.278
Calcium pantothenate 10 mg 0.05 0.105
p-Aminobenzoic acid 10 mg 0.05 0.365
D-Biotin 5 mg 0.025 0.102
Distilled water 200 ml - -

Metadata

Last modified: 24.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Polypeptone 2727.27 -
Agar 1363.64 4054.46
Glycerol 181.818 1974.35
L-Asparagine 90.909 688.08
K2HPO4 90.909 521.938
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
temperature mesophilic 1 strain