Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 0.30 | g | 0.294 | 2.161 | |
K2HPO4 | 0.30 | g | 0.294 | 1.689 | |
(NH4)2SO4 | 0.30 | g | 0.294 | 2.226 | |
NaCl | 0.60 | g | 0.588 | 10.066 | |
MgSO4 x 7 H2O | 0.13 | g | 0.127 | 0.517 | |
FeSO4 x 7 H2O | 2.00 | mg | 0.002 | 0.007 | |
CaCl2 x 2 H2O | 8.00 | mg | 0.008 | 0.053 | |
Yeast extract
(BD-Difco) |
2.00 | g | 1.961 | - | |
Trypticase peptone
(BD-BBL) |
2.00 | g | 1.961 | - | |
Sodium acetate | 2.50 | g | 2.451 | 29.879 | |
Sodium formate | 2.50 | g | 2.451 | 36.04 | |
Trace minerals | 10.00 | ml | - | - | |
Trace vitamins | 10.00 | ml | - | - | |
NaHCO3 | 5.00 | g | 4.902 | 58.352 | |
Na2S x 9 H2O | 0.50 | g | 0.49 | 2.041 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.49 | - | |
Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
Distilled water | 1000.00 | ml | - | - | |
1 Mix ingredients, except NaHCO3, L-Cysteine x HCl x H2O and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
NaCl | 1.00 | g | 1 | 17.112 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
H3BO3 | 0.01 | g | 0.01 | 0.162 | |
Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 29.10.21 |
Source: | JCM |
Taxonomic range: | Archaea |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 5 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaHCO3 | 4.902 | 58.352 |
Sodium formate | 2.451 | 36.04 |
Sodium acetate | 2.451 | 29.879 |
Trypticase peptone | 1.961 | - |
Yeast extract | 1.961 | - |
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