Name: MJ/YTCT MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J274 #

Equipment needed: Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract (BD-Difco)	2.00	g	2	-
Tryptone (BD-Difco)	2.00	g	2	28.137
Sodium tartrate	1.00	g	1	5.153
Trisodium citrate	1.00	g	1	3.875
NaNO₃	0.25	g	0.25	2.941
(NH₄)₂SO₄	0.25	g	0.25	1.892
Ferric citrate	0.20	g	0.2	0.817
MJ(-N) synthetic seawater	100.00	ml	-	-
Na₂SO₄	1.00	g	1	7.04
Na₂S₂O₃ x 5 H₂O	2.00	g	2	8.058
Na₂CO₃	40.00	g	40	377.401
Distilled water	900.00	ml	-	-
1 Adjust pH to 11.0-12.0 with KOH and autoclave under a N₂ atmosphere. After autoclaving, replace the gas phase with N₂-CO₂ (80:20) and pressurize to 200 kPa.

MJ(-N) synthetic seawater #

1010

Solution occurs in 12 other media (first defined in Medium J268).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	30.00	g	29.703	508.264
K₂HPO₄	0.14	g	0.139	0.796
CaCl₂ x 2 H₂O	0.14	g	0.139	0.943
MgSO₄ x 7 H₂O	3.40	g	3.366	13.658
MgCl₂ x 6 H₂O	4.18	g	4.139	20.357
KCl	0.33	g	0.327	4.383
NiCl₂ x 6 H₂O	0.50	mg	5.0e-4	0.004
Na₂SeO₃ x 5 H₂O	0.50	mg	5.0e-4	0.002
Fe(NH₄)₂(SO₄)₂ x 6 H₂O	0.01	g	0.01	-
Modified Wolin's mineral solution	10.00	ml	-	-
Distilled water	1000.00	ml	-	-

All strains for this medium:

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