Name: MJYP* MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J277 #

Equipment needed: Autoclave Anaerobic workbench

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract (BD-Difco)	2.00	g	2	-
Trypticase peptone (BD-BBL)	2.00	g	2	-
NaNO₃	1.70	g	1.7	20.001
FeSO₄ x 7 H₂O	1.39	g	1.39	5
Na₂S x 9 H₂O	0.50	g	0.5	2.082
Resazurin	1.00	mg	1.0e-3	0.004
MJ(-N) synthetic seawater	1000.00	ml	-	-
1 Mix ingredients, except NaNO₃, FeSO₄ x 7H2O and Na₂S x 9H2O, and autoclave under a N₂ atmosphere. Separately autoclave NaNO₃, FeSO₄ x 7H2O and Na₂S x 9H2O solutions (pH 7.0, 7.5 and 7.5, respectively) under a N₂ atmosphere. Aseptically and anaerobically add the NaNO₃, FeSO₄ x 7H2O and Na₂S x 9H2O solutions to the medium and adjust pH to 6.0. Pressurize the inoculated bottle to 200 kPa N₂.

MJ(-N) synthetic seawater #

1010

Solution occurs in 12 other media (first defined in Medium J268).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	30.00	g	29.703	508.264
K₂HPO₄	0.14	g	0.139	0.796
CaCl₂ x 2 H₂O	0.14	g	0.139	0.943
MgSO₄ x 7 H₂O	3.40	g	3.366	13.658
MgCl₂ x 6 H₂O	4.18	g	4.139	20.357
KCl	0.33	g	0.327	4.383
NiCl₂ x 6 H₂O	0.50	mg	5.0e-4	0.004
Na₂SeO₃ x 5 H₂O	0.50	mg	5.0e-4	0.002
Fe(NH₄)₂(SO₄)₂ x 6 H₂O	0.01	g	0.01	-
Modified Wolin's mineral solution	10.00	ml	-	-
Distilled water	1000.00	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Archaea
Medium type:	Complex medium
Final pH:	6.0
Equipment needed:	Anaerobic workbench, Autoclave

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