J280
280 .
THERMOCOCCUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J280 #
ml
Solution occurs in 1
other media (first defined in Medium J280).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.330 | g | 0.324 | 4.34 | |
| MgCl2 x 6 H2O | 2.800 | g | 2.745 | 13.503 | |
| MgSO4 x 7 H2O | 3.400 | g | 3.333 | 13.524 | |
| NH4Cl | 0.250 | g | 0.245 | 4.582 | |
| NaCl | 25.000 | g | 24.51 | 419.401 | |
| K2HPO4 | 0.300 | g | 0.294 | 1.689 | |
|
Yeast extract
(BD-Difco) |
3.000 | g | 2.941 | - | |
|
Tryptone
(BD-Difco) |
3.000 | g | 2.941 | 41.378 | |
| FeSO4 x 7 H2O | 0.025 | g | 0.025 | 0.088 | |
| NaBr | 10.000 | mg | 0.01 | 0.095 | |
| Trace minerals | 10.000 | ml | - | - | |
| Trace vitamins | 10.000 | ml | - | - | |
|
Sulfur
(powder) |
10.000 | g | 9.804 | 305.704 | |
| Na2S x 9 H2O | 0.500 | g | 0.49 | 2.041 | |
| Resazurin | 1.000 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Mix ingredients except sulfur and Na2S x 9H2O, adjust pH to 7.0-7.2 and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution and autoclave under a N2 gas atmosphere. Distribute the medium and sulfur into culture vessels under a N2 stream, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 7.0-7.2, if necessary. | |||||
| 2 Comment: | |||||
| 3 Most of Thermococcus strains will grow in this medium supplemented with 5.0 g/L maltodextrin and omitted sulfur. | |||||
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.1 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 26 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 24.52 | 419.569 |
| Sulfur | 9.804 | 305.704 |
| MgSO4 x 7 H2O | 3.363 | 13.643 |
| Tryptone | 2.941 | 41.378 |
| Yeast extract | 2.941 | - |
| Show more | ||
Cultivation metadata from 26 strains
| pH | 6 - 8 | 1 strain |
| phyla | 2 | 26 strains |
| oxygen | anaerobe | 18 strains |
| temperature | hyperthermophilic | 24 strains |
| temperature | thermophilic | 2 strains |