MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 320 . THERMOTOGA YE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J320 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(BD-Difco) 		2.000 g 1.961 -
NaCl 20.000 g 19.608 335.521
MgCl2 x 6 H2O 3.000 g 2.941 14.467
MgSO4 x 7 H2O 6.000 g 5.882 23.866
(NH4)2SO4 1.000 g 0.98 7.419
CaCl2 x 2 H2O 0.300 g 0.294 2.001
KH2PO4 0.200 g 0.196 1.441
KCl 0.500 g 0.49 6.575
NaBr 0.050 g 0.049 0.476
H3BO3 0.025 g 0.025 0.396
SrCl2 x 6 H2O 0.020 g 0.02 0.074
Ferric citrate 0.010 g 0.01 0.04
Trace minerals 10.000 ml - -
Trace vitamins 10.000 ml - -
Na2S x 9 H2O 0.500 g 0.49 2.041
Resazurin 1.000 mg 9.8e-4 0.004
Distilled water 1000.000 ml - -
1 Mix ingredients, except yeast extract and Na2S x 9H2O, adjust pH to 6.5 with H2SO4, and filter-sterilize under a N2 atmosphere. Separately autoclave 10% yeast extract solution and neutralized 5% Na2S x 9H2O solution under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution to the medium. Prior to inoculation, add the Na2S x 9H2O solution to the medium, and readjust pH to 6.5 - 7.0, if necessary. Cultivate under a N2 atmosphere.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.618 335.689
MgSO4 x 7 H2O 5.912 23.985
MgCl2 x 6 H2O 2.941 14.467
Yeast extract 1.961 -
(NH4)2SO4 0.98 7.419
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
nutrition heterotroph 1 strain
Show more