J421
421 .
MMJHS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J421 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MJ(-N) synthetic seawater | 1000 | ml | - | - | |
| NaNO3 | 1 | g | 0.99 | 11.649 | |
| Na2S2O3 x 5 H2O | 1 | g | 0.99 | 3.989 | |
| NaHCO3 | 1 | g | 0.99 | 11.786 | |
| Trace vitamins | 10 | ml | - | - | |
|
Sulfur
(powder) |
3 | g | 2.97 | 92.619 | |
| 1 Mix ingredients except sulfur, adjust pH to 6.5 - 7.0 with NaOH and filter-sterilize. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v). | |||||
| 2 Comment: Adjust final pH to 7.0 (for strain JCM 12458) or 6.5 (for strain JCM 12459). | |||||
Solution occurs in 12
other media (first defined in Medium J268).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 30.00 | g | 29.703 | 508.264 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.14 | g | 0.139 | 0.943 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| MgCl2 x 6 H2O | 4.18 | g | 4.139 | 20.357 |
| KCl | 0.33 | g | 0.327 | 4.383 |
| NiCl2 x 6 H2O | 0.50 | mg | 5.0e-4 | 0.004 |
| Na2SeO3 x 5 H2O | 0.50 | mg | 5.0e-4 | 0.002 |
| Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - |
| Modified Wolin's mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 29.419 | 503.399 |
| MgCl2 x 6 H2O | 4.098 | 20.155 |
| MgSO4 x 7 H2O | 3.362 | 13.642 |
| Sulfur | 2.97 | 92.619 |
| NaHCO3 | 0.99 | 11.786 |
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