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J421. MMJHS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 MJ(-N) synthetic seawater 1000 ml - -
2 1 g 0.99 11.649
3 1 g 0.99 -
4 1 g 0.99 11.786
5 Trace vitamins 10 ml - -
6
(powder)
3 g 2.97 92.631
Instructions:
  1. Mix ingredients except sulfur, adjust pH to 6.5 - 7.0 with NaOH and filter-sterilize. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v).
  2. Comment: Adjust final pH to 7.0 (for strain JCM 12458) or 6.5 (for strain JCM 12459).
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 10 other media (first defined in medium J268 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 29.703 508.243
2 0.14 g 0.139 0.796
3 0.14 g 0.139 0.943
4 3.40 g 3.366 13.658
5 4.18 g 4.139 20.357
6 0.33 g 0.327 4.383
7 0.50 mg 5.0e-4 -
8 0.50 mg 5.0e-4 -
9 0.01 g 0.01 -
10 Trace minerals 10.00 ml - -
11 1000.00 ml 988.324 54860.2

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 29.419 503.379
MgCl2 x 6H2O 4.098 20.156
MgSO4 x 7H2O 3.362 13.642
Sulfur 2.97 92.631
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