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JCM 601 . ANCALOMICROBIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Devosia enhydra DSM 8908

This strain uses the standard medium without modifications.

Main sol. J601 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.250 g 0.248 1.873
Glucose 0.250 g 0.248 1.374
Vitamin solution 10.000 ml - -
Modified Hutner's basal salts 20.000 ml - -
Na2HPO4 0.071 g 0.07 0.495
Distilled water 980.000 ml - -
1 Adjust pH to 7.0.
2 Separately sterilize Vitamin solution by filtration and aseptically add to the medium.

Vitamin solution #

1000
Solution occurs in 1 other media (first defined in Medium J979).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
Biotin 2.0 mg 0.002 0.008
Thiamine-HCl x 2 H2O 5.0 mg 0.005 -
Calcium pantothenate 5.0 mg 0.005 0.01
Folic acid 2.0 mg 0.002 0.005
Riboflavin 5.0 mg 0.005 0.013
Nicotinamide 5.0 mg 0.005 0.041
Distilled water 1000.0 ml - -
1 Filter-sterilize.

Modified Hutner's basal salts #

1000
Solution occurs in 3 other media (first defined in Medium J900).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA) 		10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metals ''44'' 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.

Metals ''44'' #

1000
Solution occurs in 7 other media (first defined in Medium J149).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 250.0 mg 0.25 -
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x n H2O 154.0 mg 0.154 -
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 2
Devosia enhydra DSM 8908

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.588 2.386
(NH4)2SO4 0.248 1.873
Glucose 0.248 1.374
Nitrilotriacetic acid 0.198 1.036
Na2HPO4 0.07 0.495
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains