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JCM 728 . DESULFOSPOROSINUS ACIDIPHILUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J728 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
HBS solution 20.00 ml - -
Trace minerals 10.00 ml - -
Ni-Se-W solution 10.00 ml - -
K2SO4 0.87 g 0.87 4.993
Yeast extract 0.10 g 0.1 -
Distilled water 939.00 ml - -
1 Mix thoroughly, adjust to pH 5.5 with H2SO4 and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solutions (filter-sterilized) to the medium:
Glycerol
(1 M) 		4.00 ml - -
FeSO4
(pH 2.0, 10 mM) 		10.00 ml - -
ZnSO4
(1 M) 		7.00 ml - -

HBS solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 3.3 g 3.3 23.233
(NH4)2SO4 22.5 g 22.5 170.274
KCl 2.5 g 2.5 33.535
MgSO4 x 7 H2O 25.0 g 25 101.43
K2HPO4 2.5 g 2.5 14.353
Ca(NO3)2 x 4 H2O 0.7 g 0.7 2.964
Distilled water 1000.0 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Ni-Se-W solution #

1000
Solution occurs in 3 other media (first defined in Medium J244).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
(NH4)2Ni(SO4)2 x 6 H2O 2.0 g 2 5.063
Na2SeO3 0.3 mg 3.0e-4 -
Na2SeO4 10.0 mg 0.01 0.053
Na2WO4 x 2 H2O 10.0 mg 0.01 0.03
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 5.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
K2SO4 0.87 4.993
MgSO4 x 7 H2O 0.53 2.15
(NH4)2SO4 0.45 3.405
Yeast extract 0.1 -
Na2SO4 0.066 0.465
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