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JCM 729 . ACIDITHIOBACILLUS FERRIVORANS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J729 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
UBS solution 100 ml - -
Trace minerals 10 ml - -
Ni-Se-W solution 10 ml - -
Distilled water 845 ml - -
1 Mix thoroughly, adjust pH to 2.5 with H2SO4 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
K2S4O6
(0.1 M) 		25 ml - -
FeSO4
(pH 2.0, 10 mM) 		10 ml - -

UBS solution #

1000
Solution occurs in 1 other media (first defined in Medium J729).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 14.00 g 14 98.564
(NH4)2SO4 30.00 g 30 227.032
KCl 1.00 g 1 13.414
MgSO4 x 7 H2O 5.00 g 5 20.286
K2HPO4 0.50 g 0.5 2.871
Ca(NO3)2 x 4 H2O 0.14 g 0.14 0.593
Distilled water 1000.00 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Ni-Se-W solution #

1000
Solution occurs in 3 other media (first defined in Medium J244).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NiCl2 x 6 H2O 25.0 mg 0.025 0.193
(NH4)2Ni(SO4)2 x 6 H2O 2.0 g 2 5.063
Na2SeO3 0.3 mg 3.0e-4 -
Na2SeO4 10.0 mg 0.01 0.053
Na2WO4 x 2 H2O 10.0 mg 0.01 0.03
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 2.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 3 22.703
Na2SO4 1.4 9.856
MgSO4 x 7 H2O 0.53 2.15
KCl 0.1 1.341
K2HPO4 0.05 0.287
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain