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JCM 772 . SALTWATER MEDIUM WITH HYDROGEN

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J772 #

ml
Equipment needed: Autoclave Anaerobic workbench Filter
1 Mix components thoroughly, bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in Balch tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.00 g 18.975 324.698
Na2SO4 4.00 g 3.795 26.718
MgCl2 x 6 H2O 3.00 g 2.846 14
KCl 0.50 g 0.474 6.363
CaCl2 x 2 H2O 0.15 g 0.142 0.968
NH4Cl 0.25 g 0.237 4.434
KH2PO4 0.20 g 0.19 1.394
Resazurin 1.00 mg 9.5e-4 0.004
Distilled water 1000.00 ml - -
Trace element solution 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Vitamin solution (see Medium No. 403)
(*) 		1.00 ml - -
Thiamine solution (see Medium No. 403)
(*) 		1.00 ml - -
Vitamin B12 solution (see Medium No. 403)
(*) 		1.00 ml - -
Riboflavin solution (see Medium No. 462)
(*) 		1.00 ml - -
Sodium fumarate
(1.0 M) 		10.00 ml - -
NaHCO3
(*, 8%) 		30.00 ml - -
Na2S x 9 H2O
(5%) 		8.00 ml - -
2 Readjust pH of the medium to 7.2 - 7.4, if necessary. Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).

Trace element solution #

1000
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 18.975 324.698
Na2SO4 3.795 26.718
MgCl2 x 6 H2O 2.846 14
KCl 0.474 6.363
NH4Cl 0.237 4.434
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
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