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JCM 817 . LOW-SALT METHANOTROPHIC MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J817 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 0.10 g 0.1 0.988
MgSO4 x 7 H2O 0.10 g 0.1 0.405
CaCl2 x 2 H2O 0.02 g 0.02 0.136
KBr 0.10 g 0.1 0.839
Trace element solution 0.10 ml - -
Iron stock solution 4.5 g/L 0.10 ml - -
Distilled water 1000.00 ml - -
1 Mix components and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium:
Vitamin solution
(*) 		0.10 ml - -
Phosphate buffer stock solution 1.00 ml - -
Selenite-tungstate solution 0.10 ml - -
2 To complete medium, adjust pH to 6.8. Then, distribute 20 ml-aliquots of the medium into sterile 120 ml-serum bottles and seal with butyl rubber stoppers. Add 100 ml methane gas (filter-sterilized) into the head space of the culture bottle through a syringe.

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 10 mg 0.01 -
Nicotinic acid 20 mg 0.02 0.162
Pyridoxine hydrochloride 10 mg 0.01 -
p-Aminobenzoic acid 10 mg 0.01 0.073
Riboflavin 20 mg 0.02 0.053
Biotin 1 mg 1.0e-3 0.004
Vitamin B12 5 mg 0.005 0.004
Distilled water 1000 ml - -

Trace element solution #

1000
Solution occurs in 1 other media (first defined in Medium J815).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.400 g 0.4 1.391
Na2-EDTA 0.250 g 0.25 -
MnCl2 x 4 H2O 0.020 g 0.02 -
H3BO3 0.015 g 0.015 0.243
Na2MoO4 x 2 H2O 0.040 g 0.04 0.165
NiCl2 x 6 H2O 0.010 g 0.01 0.077
CuSO4 x 5 H2O 0.200 g 0.2 0.801
CoCl2 x 6 H2O 0.050 g 0.05 0.385
Distilled water 1000.000 ml - -

Iron stock solution 4.5 g/L #

1000
Solution occurs in 1 other media (first defined in Medium J815).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III)-EDTA 4.5 g 4.5 -
Distilled water 1000.0 ml - -

Phosphate buffer stock solution #

1000
Solution occurs in 1 other media (first defined in Medium J815).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 37.425 g 37.425 275.01
Na2HPO4 x 2 H2O 48.950 g 48.95 344.818
Distilled water 1000.000 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 71 other media (first defined in Medium J431).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.4 g 0.4 10.001
Na2SeO3 x 5 H2O 6.0 mg 0.006 0.023
Na2WO4 x 2 H2O 8.0 mg 0.008 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KNO3 0.1 0.988
KBr 0.1 0.839
MgSO4 x 7 H2O 0.1 0.405
Na2HPO4 x 2 H2O 0.049 0.344
KH2PO4 0.037 0.275
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