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JCM 865 . DESULFOSPOROSINUS BSREI1 MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.450 g 0.446 3.375
KH2PO4 0.050 g 0.05 0.364
MgSO4 x 7 H2O 0.500 g 0.496 2.011
KCl 0.050 g 0.05 0.665
Ca(NO3)2 x 4 H2O 0.014 g 0.014 0.059
Na2SO4 3.000 g 2.973 20.932
Trace element solution 1.000 ml - -
Yeast extract 0.500 g 0.496 -
L-Cysteine HCl x H2O 0.500 g 0.496 -
Resazurin 1.000 mg 9.9e-4 0.004
Distilled water 910.000 ml - -
1 Mix components thoroughly, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 910 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 5%)
60.000 ml - -
Fructose
(1 M)
20.000 ml - -
Trace vitamins (see Medium No. 197)
(*)
10.000 ml - -
Na2S x 9 H2O
(5%)
8.000 ml - -
Solution occurs in 38 other media (first defined in Medium J439).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
12.5 ml - -
FeSO4 x 7 H2O 2.1 g 2.1 7.554
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 -
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 987.0 ml - -

Metadata

Media status: Inofficial medium
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.973 20.932
MgSO4 x 7 H2O 0.496 2.011
Yeast extract 0.496 -
L-Cysteine HCl x H2O 0.496 -
(NH4)2SO4 0.446 3.375
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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