Name: GEOMONAS SACCHAROVORANS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J868 #

Equipment needed: Autoclave Anaerobic workbench Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	0.3	g	0.298	2.189
K₂HPO₄	0.3	g	0.298	1.71
NH₄Cl	1.0	g	0.993	18.565
NaCl	1.0	g	0.993	16.993
KCl	0.1	g	0.099	1.332
CaCl₂ x 2 H₂O	0.1	g	0.099	0.675
Na₂SO₄	3.0	g	2.979	20.974
Sodium acetate	1.0	g	0.993	12.106
Trace element solution	1.0	ml	-	-
Yeast extract	1.0	g	0.993	-
L-Cysteine HCl x H₂O	0.5	g	0.497	-
Resazurin	0.5	mg	5.0e-4	0.002
Distilled water	950.0	ml	-	-
1 Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 950 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	25.0	ml	-	-
Fructose (1 M)	20.0	ml	-	-
Vitamin solution (see Medium No. 403) (*)	1.0	ml	-	-
Thiamine solution (see Medium No. 403) (*)	1.0	ml	-	-
Vitamin B₁₂ solution (see Medium No. 403) (*)	1.0	ml	-	-
Na₂S x 9 H₂O (5%)	8.0	ml	-	-

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Medium type:	Complex medium
Final pH:	7.2
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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All strains for this medium:

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Compound	Conc. [g/L]	Conc. [mM]
Na₂SO₄	2.979	20.974
NH₄Cl	0.993	18.565
NaCl	0.993	16.993
Sodium acetate	0.993	12.106
Yeast extract	0.993	-
L-Cysteine HCl x H₂O	0.497	-
KH₂PO₄	0.298	2.189
K₂HPO₄	0.298	1.71
KCl	0.099	1.332
CaCl₂ x 2 H₂O	0.099	0.675
FeSO₄ x 7 H₂O	0.002	0.008
Resazurin	5.0e-4	0.002
CoCl₂ x 6 H₂O	1.9e-4	0.001
ZnSO₄ x 7 H₂O	1.4e-4	5.0e-4
MnCl₂ x 4 H₂O	9.9e-5	-
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
H₃BO₃	3.0e-5	4.8e-4
NiCl₂ x 6 H₂O	2.4e-5	1.8e-4
CuCl₂ x 2 H₂O	2.0e-6	1.2e-5
Distilled water	-	-
Vitamin B₁₂ solution (see Medium No. 403)	-	-
Thiamine solution (see Medium No. 403)	-	-
Vitamin solution (see Medium No. 403)	-	-
Fructose	-	-
NaHCO₃	-	-
HCl	-	-
Na₂S x 9 H₂O	-	-
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