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JCM 898 . SYPHC MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J898 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sea Salt
(Sigma) 		35.00 g 34.93 -
Wolfe's mineral elixir 1.00 ml - -
NH4Cl 0.10 g 0.1 1.866
Yeast extract 1.00 g 0.998 -
Sodium pyruvate 1.10 g 1.098 9.976
HEPES 2.50 g 2.495 10.47
L-Histidine 0.04 g 0.04 0.257
L-Cysteine HCl x H2O 0.04 g 0.04 -
Vitamin solution 0.50 ml - -
Distilled water 1000.00 ml - -
1 Mix components thoroughly and adjust pH to 7.7. After autoclaving, add the following solution (autoclaved):
KH2PO4
(5%) 		1.00 ml - -

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium J616).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
Biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
DL-Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 -
Thiamine HCl 200 mg 0.2 -
Distilled water 1000 ml - -

Wolfe's mineral elixir #

1000
Solution occurs in 8 other media (first defined in Medium J470).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 30.00 g 30 121.716
MnSO4 x n H2O 5.00 g 5 -
NaCl 10.00 g 10 171.116
FeSO4 x 7 H2O 1.00 g 1 3.597
CoCl2 x 6 H2O 1.80 g 1.8 13.863
CaCl2 x 2 H2O 1.00 g 1 6.802
ZnSO4 x 7 H2O 1.80 g 1.8 6.26
CuSO4 x 5 H2O 0.10 g 0.1 0.401
AlK(SO4)2 x 12 H2O 0.18 g 0.18 -
H3BO3 0.10 g 0.1 1.617
Na2MoO4 x 2 H2O 0.10 g 0.1 0.413
(NH4)2Ni(SO4)2 x 6 H2O 2.80 g 2.8 7.089
Na2WO4 x 2 H2O 0.10 g 0.1 0.303
Na2SeO4 0.10 g 0.1 0.529
Distilled water 1000.00 ml - -
1 First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.7
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sea Salt 34.93 -
HEPES 2.495 10.47
Sodium pyruvate 1.098 9.976
Yeast extract 0.998 -
NH4Cl 0.1 1.866
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain