Name: CEJFB1T MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J987 #

Equipment needed: Anaerobic workbench Filter Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.0	g	0.991	18.528
K₂HPO₄	0.5	g	0.496	2.845
CaCl₂ x 2 H₂O	0.1	g	0.099	0.674
KCl	0.8	g	0.793	10.635
Sodium acetate	0.5	g	0.496	6.041
NaCl	200.0	g	198.216	3391.79
Yeast extract (BD-Difco)	1.0	g	0.991	-
Tryptone (BD-Difco)	0.5	g	0.496	6.972
Trace element solution	1.0	ml	-	-
L-Cysteine HCl x H₂O	0.5	g	0.496	-
Resazurin	0.5	mg	5.0e-4	0.002
Distilled water	855.0	ml	-	-
1 Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO₃ (*, 8%)	25.0	ml	-	-
Glucose (1 M)	20.0	ml	-	-
MgSO₄ x 7 H₂O (10%)	50.0	ml	-	-
Tryptone (10%)	50.0	ml	-	-
2 Aseptically distribute the medium into culture vessels under a stream of N₂-CO₂ (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N₂ atmosphere).
Na₂S x 9 H₂O (5%)	8.0	ml	-	-

Trace element solution #

1000

Solution occurs in 38 other media (first defined in Medium J439).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
HCl (25%)	12.5	ml	-	-
FeSO₄ x 7 H₂O	2.1	g	2.1	7.554
H₃BO₃	30.0	mg	0.03	0.485
MnCl₂ x 4 H₂O	100.0	mg	0.1	-
CoCl₂ x 6 H₂O	190.0	mg	0.19	1.463
NiCl₂ x 6 H₂O	24.0	mg	0.024	0.185
CuCl₂ x 2 H₂O	2.0	mg	0.002	0.012
ZnSO₄ x 7 H₂O	144.0	mg	0.144	0.501
Na₂MoO₄ x 2 H₂O	36.0	mg	0.036	0.149
Distilled water	987.0	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	7.6
Equipment needed:	Anaerobic workbench, Autoclave, Filter

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