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J994. MBM MEDIUM 2

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 80.0 g 78.278 1339.4
2 6.0 g 5.871 23.819
3 1.5 g 1.468 19.687
4 1.0 g 0.978 18.292
5 0.4 g 0.391 2.662
6 0.4 g 0.391 2.247
7 Trace minerals 10.0 ml - -
8 1.6 g 1.566 -
9 1.0 mg 9.8e-4 0.004
10 900.0 ml 879.047 48794.5
11
(1 M)
40.0 ml - -
12
(*, 8%)
50.0 ml 8.45 100.589
13 Trace vitamins
(*)
10.0 ml - -
14
(5%)
6.0 ml - -
15
(5%)
6.0 ml - -
Instructions:
  1. Mix components thoroughly. Bring to a boil for a few seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized): #11-13
  2. Aseptically distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 gas atmosphere): #14-15
  3. Readjust pH to 7.5, if necessary.
Solution occurs in 88 other media (first defined in medium J151 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.171
3 0.50 g 0.5 -
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.10 g 0.1 -
7 0.10 g 0.1 0.68
8 0.10 g 0.1 -
9 0.01 g 0.01 -
10 0.01 g 0.01 0.039
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Solution occurs in 218 other media (first defined in medium J197 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 -
4 5.0 mg 0.005 -
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 -
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Inofficial medium
Source: JCM
Medium type: Complex medium
Final pH: unknown

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 898.581 49878.8
NaCl 78.288 1339.57
NaHCO3 8.45 100.589
MgSO4 x 7H2O 5.9 23.938
Sodium acetate x 3H2O 1.566 -
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