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Trace element solution

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Internal Solution-ID: S1494

This solution is used in 5 media and was first defined in medium 732.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.5 g 0.5 1.487
FeCl2 x 4 H2O 2.0 g 2 10.06
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
AlCl3 10.0 mg 0.01 0.075
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 First dissolve EDTA in distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add remaining compounds.
727 CLOSTRIDIUM ULTUNENSE MEDIUM
732 DEHALOBACTER RESTRICTUS MEDIUM (TCE)
732a DESULFUROMONAS MEDIUM (TCE)
1271 PLEOMORPHOCHAETA MEDIUM
1301 TEPIDANAEROBACTER ACETATOXYDANS MEDIUM