Solution 1
Internal Solution-ID: S3200
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Hutner's salts | 20 | ml | - | - | |
|
Tris-HCl buffer
(0.1M, pH 7.5) |
50 | ml | - | 5 | |
| Gelrite | 8 | g | 8.333 | - | |
| MgCl2 | 1 | g | 1.042 | 10.941 | |
|
Sea water
(Biomaris ) |
700 | ml | - | - | |
| Distilled water | 190 | ml | - | - | |
| 1 Adjust pH to 7.5, autoclave at 121°C for 20 min. | |||||
Media with this solution
| 1544 | . PIRELLULA MEDIUM (M30) |