DSMZ 1544 . . PIRELLULA MEDIUM (M30)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 950 ml - -
Solution 2 50 ml - -
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Hutner's salts 20 ml - -
Tris-HCl buffer
(0.1M, pH 7.5)
50 ml - 5
Gelrite 8 g 8.333 -
MgCl2 1 g 1.042 10.941
Sea water
(Biomaris )
700 ml - -
Distilled water 190 ml - -
1 Adjust pH to 7.5, autoclave at 121°C for 20 min.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
N-Acetylglucosamine 2.00 g 40 180.824
Na2HPO4 x 2 H2O 0.01 g 0.2 1.409
Vitamin solution No.6 10.00 ml - -
Distilled water 40.00 ml - -
1 Adjust pH to 7.5, filter-sterilize.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
Biotin 4.0 mg 0.004 0.016
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Calcium pantothenate 10.0 mg 0.01 0.021
Folic acid 4.0 mg 0.004 0.009
Riboflavin 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Pyridoxine hydrochloride 20.0 mg 0.02 0.097
Distilled water 1000.0 ml - -
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metall salt sol. 44 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Gelrite 7.917 -
N-Acetylglucosamine 2 9.041
MgCl2 0.99 10.394
MgSO4 x 7 H2O 0.588 2.385
Nitrilotriacetic acid 0.198 1.035
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain