Main sol. J1097
Internal Solution-ID: S5171
Equipment needed: Anaerobic workbench
Filter
Autoclave
| 1 Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 19.608 | 335.521 | |
| MgCl2 x 6 H2O | 3.00 | g | 2.941 | 14.467 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.147 | 1 | |
| NH4Cl | 0.25 | g | 0.245 | 4.582 | |
| KH2PO4 | 0.20 | g | 0.196 | 1.441 | |
| KCl | 0.50 | g | 0.49 | 6.575 | |
| RST trace elements | 10.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 922.50 | ml | - | - | |
|
NaHCO3
(*, 8%) |
37.50 | ml | - | - | |
|
RST vitamin solution
(*) |
10.00 | ml | - | - | |
|
Sodium pyruvate
(1 M) |
20.00 | ml | - | - | |
|
Yeast extract
(1%) |
10.00 | ml | - | - | |
| 2 Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solution: | |||||
| Reducing agent solution | 10.00 | ml | - | - | |
Media with this solution
| J1097 | SEAWATER PYRUVATE THIOSULFATE MEDIUM |