Main sol. 377a
Internal Solution-ID: S718
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 30.00 | g | 29.97 | 512.834 | |
MgSO4 x 7 H2O | 3.50 | g | 3.497 | 14.186 | |
MgCl2 x 6 H2O | 2.75 | g | 2.747 | 13.513 | |
KCl | 0.33 | g | 0.33 | 4.422 | |
NaBr | 0.05 | g | 0.05 | 0.485 | |
H3BO3 | 15.00 | mg | 0.015 | 0.242 | |
SrCl2 x 6 H2O
(0.1% w/v) |
7.00 | ml | 0.007 | 0.026 | |
(NH4)2SO4 | 10.00 | mg | 0.01 | 0.076 | |
Citric acid
(0.1% w/v) |
5.00 | ml | 0.005 | 0.026 | |
KI
(0.01% w/v) |
0.50 | ml | 5.0e-5 | 1.9e-4 | |
CaCl2 x 2 H2O | 0.75 | g | 0.749 | 5.096 | |
KH2PO4 | 0.50 | g | 0.5 | 3.671 | |
NiCl2 x 6 H2O
(0.1% w/v) |
2.00 | ml | 0.002 | 0.015 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Sulfur
(powdered) |
30.00 | g | 29.97 | 934.519 | |
Peptone
(BD Bacto) |
5.00 | g | 4.995 | - | |
Yeast extract
(OXOID) |
1.00 | g | 0.999 | - | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Neutralized sulfide solution 3% (w/v) | 15.00 | ml | - | - | |
Distilled water | 960.00 | ml | - | - | |
1 Dissolve ingredients except sulfur, peptone, yeast extract, vitamins, and sulfide, adjust the pH to 6.5 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials that already contain the appropriate amount of sulfur. Sterilize the medium by autoclaving at 110°C for 20 min. After sterilization add peptone, yeast extract, and vitamins from sterile stock solutions prepared under 100% N2 gas atmosphere and sulfide from a neutralized stock solution. Vitamins should be sterilized by filtration. |
377a | PALAEOCOCCUS MEDIUM |