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Current criteria: 2-Mercaptoethanesulfonate

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120b METHANOMICROCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaHCO3 2.441 29.062
NaCl 2.197 37.599
Yeast extract 1.953 -
Casitone 1.953 -
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
L-Cysteine HCl x H2O 0.352 2.002
Na2S x 9 H2O 0.352 1.464
K2HPO4 0.342 1.962
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
2-Mercaptoethanesulfonate 0.137 0.961
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

479 METHANOHALOPHILUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 6 29.513
Trypticase peptone 2 -
Yeast extract 2 -
Trimethylamine-HCl 2 -
KCl 1.5 20.121
Na2CO3 1.5 14.153
NH4Cl 1 18.695
K2HPO4 x 3 H2O 0.4 2.297
Na2S x 9 H2O 0.25 1.041
2-Mercaptoethanesulfonic acid 0.2 1.406
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Solutions:
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % N2
  • 20 % CO2

479a METHANOHALOPHILUS PROFUNDI MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 6 29.513
HEPES 5 20.982
Trypticase peptone 2 -
Yeast extract 2 -
Trimethylamine-HCl 2 -
KCl 1.5 20.121
NH4Cl 1 18.695
K2HPO4 x 3 H2O 0.4 2.297
Na2S x 9 H2O 0.25 1.041
2-Mercaptoethanesulfonic acid 0.2 1.406
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Solutions:
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 100 % N2

490 METHANOLOBUS OREGONENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 2.5 23.588
Trimethylamine-HCl 2 -
Yeast extract 2 -
Trypticase peptone 2 -
MgCl2 x 6 H2O 1.7 8.362
KCl 1.5 20.121
NH4Cl 1 18.695
2-Mercaptoethanesulfonic acid 0.5 3.516
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Solutions:
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 100 % N2

705 BACILLUS INFERNUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 5.631 96.356
Ferric citrate monohydrate 5.146 19.568
Yeast extract 1.942 -
Trypticase peptone 1.942 -
Na-lactate 1.165 10.397
NH4Cl 0.971 18.151
MgCl2 x 6 H2O 0.971 4.776
Na-acetate 0.777 9.468
2-Mercaptoethanesulfonic acid 0.485 3.414
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Solutions:
  • 10 ml Trace element solution
Gas phase:
  • 80 % N2
  • 20 % CO2

1030 SMITHELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.957 -
Trypticase peptone 1.957 -
Crotonic acid 1.683 19.549
NH4Cl 0.978 18.293
Na2CO3 0.978 9.232
MgCl2 x 6 H2O 0.978 4.813
2-Mercaptoethanesulfonic acid 0.489 3.44
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Solutions:
  • 1 ml Wolfe's mineral elixir
  • 20 ml Na-crotonate solution (1 M)
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

1084 METHERMICOCCUS (METHANOGEN) MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 23.506 402.231
Methanol 12.411 387.347
MgCl2 x 6 H2O 9.99 49.14
2-Mercaptoethanesulfonic acid 2.449 17.219
Na2CO3 0.979 9.241
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Solutions:
  • 5 ml Sludge fluid
Gas phase:
  • 80 % N2
  • 20 % CO2

1094 METHANOSPHAERULA (PEAT) MEDIUM

Compound Conc. [g/L] Conc. [mM]
2-(N-Morpholino)ethanesulfonic acid 1.936 9.918
Na-acetate 0.407 4.963
NaOH 0.199 4.966
TiCl3 0.082 0.531
2-Mercaptoethanesulfonic acid 0.079 0.559
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Solutions:
  • 943 ml Solution A
  • 13 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 10 ml Solution G
  • 1 ml Trace element solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 40 % H2
  • 40 % N2
  • 20 % CO2

1098 ARCHAEOGLOBUS INFECTUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sea Salt 34.93 -
Na2S2O3 x 5 H2O 2.495 10.053
NaHCO3 1.996 23.76
Na-acetate 1.597 19.465
DL-Dithiothreitol 0.299 1.94
2-Mercaptoethanesulfonic acid 0.14 0.983
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Solutions:
  • 1 ml Wolfe's mineral elixir
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % H2
  • 20 % CO2

1280 METHANOREGULA (PEAT) MEDIUM

Compound Conc. [g/L] Conc. [mM]
Homo-PIPES 1.569 -
Yeast extract 0.199 -
TiCl3 0.082 0.531
2-Mercaptoethanesulfonate 0.07 0.489
NH4Cl 0.027 0.501
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Solutions:
  • 954 ml Solution A
  • 10 ml Solution B
  • 13 ml Solution C
  • 10 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace elements solution
  • 10 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2
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