DSMZ 1014 . THIOALKALIVIBRIO HALOPHILUS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 175.00 g 174.825 2991.53
K2HPO4 1.50 g 1.499 8.603
NH4Cl 0.50 g 0.5 9.338
Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
MgCl2 x 6 H2O 0.20 g 0.2 0.983
MgSO4 x 7 H2O 0.25 g 0.25 1.013
Na2S2O3 5.00 g 4.995 11.657
NaHCO3 4.00 g 3.996 47.568
Distilled water 1000.00 ml - -
1 Dissolve sodium chloride, hydrogenphosphate, ammonium chloride and trace elements and fill solution in screw capped Erlenmeyer flasks to 10% of volume. Autoclave at 110°C for 30 min. Add magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5.
Solution occurs in 27 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 8.0 - 8.5
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 174.825 2991.53
Na2S2O3 4.995 11.657
NaHCO3 3.996 47.568
K2HPO4 1.499 8.603
NH4Cl 0.5 9.338
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain