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1036. HUNGATEICLOSTRIDIUM ALKALICELLULOSI MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.499 9.329
2 0.2 g 0.2 1.467
3 0.1 g 0.1 0.491
4 0.2 g 0.2 2.677
5 Trace element solution SL-10 1.0 ml - -
6 Selenite-tungstate solution 1.0 ml - -
7 1.0 g 0.998 9.416
8 7.6 g 7.585 90.289
9 10.0 g 9.98 170.767
10 0.2 g 0.2 -
11 3.0 g 2.994 8.747
12 0.5 g 0.499 2.078
13 1000.0 ml 996.215 55298.2
Instructions:
  1. Dissolve ingredients except cellobiose and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas sterilized by filtration and sulfide from a sterile anoxic stock solution autoclaved under 100% N2 gas. Adjust pH of the complete medium to pH 8.8-9.0.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
NaCl 9.98 170.767
NaHCO3 7.585 90.289
Cellobiose 2.994 8.747
Na2CO3 0.998 9.416
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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