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1101. DESULFONATRONOSPIRA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 15.00 g 14.822 253.619
2 0.50 g 0.494 2.837
3 15.00 g 14.822 176.44
4 95.00 g 93.874 885.699
5 0.20 g 0.198 3.695
6 0.20 g 0.198 0.972
7 Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9 0.05 g 0.049 -
10 Vitamin solution 10.00 ml - -
11 2.20 g 2.174 19.4
12 1.30 g 1.285 10.192
13 0.24 g 0.237 0.987
14 1000.00 ml 986.371 54751.8
15 , might need strain adjustment 5.00 g/l - -
16 , might need strain adjustment 2.20 g/l - -
17 , might need strain adjustment 2.80 g/l - -
18 , might need strain adjustment 0.16 g/l - -
19 , might need strain adjustment 3.40 g/l - -
Instructions:
  1. Dissolve sodium chloride and hydrogenphospate, then sparge solution with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonates, adjust pH to 10, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, trace elements, yeast extract, vitamins, lactate, sulfite and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. Stock solutions of sulfite (freshly prepared) and vitamins should be sterilized by filtration.
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 3
Strains with modifications: 3

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2CO3 93.874 885.699
NaCl 14.822 253.619
NaHCO3 14.822 176.44
Na-L-lactate 2.174 19.4
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Cultivation metadata from 3 strains BacDive

halophily extreme 1 strain
halophily moderate 1 strain
pH 10 - 14 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
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