1245
1245 .
OTEB-MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Treponema pedis DSM 18691
* Modification: Plus 10% fetal bovine serum; anaerobic (85% N2; 10% H2; 5% CO2)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 37 °C
Main sol. 1245 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Proteose peptone no. 2 | 5.0 | g | 5 | - | |
| Heart Infusion Broth | 5.0 | g | 5 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| Potassium Phosphate , dibasic | 2.0 | g | 2 | 14.697 | |
| Sodium Chloride | 5.0 | g | 5 | 85.558 | |
| Magnesium sulfate | 0.1 | g | 0.1 | 0.831 | |
| Acetic acid | 1.6 | ml | - | - | |
| Propionic acid | 0.6 | ml | - | - | |
| n-Butyric acid | 0.4 | ml | - | - | |
| n-Valeric acid | 0.1 | ml | - | - | |
| Isovaleric acid | 0.1 | ml | - | - | |
| Isobutyric acid | 0.1 | ml | - | - | |
| 2-Methylbutyric acid | 0.1 | ml | - | - | |
|
Vitamin K1
(1% soln) |
0.1 | ml | 1.0e-3 | 0.002 | |
| L-Cysteine | 0.5 | g | 0.5 | 4.127 | |
| Fetal bovine serum , inactivated | 100.0 | ml | - | - | |
| Sugar Stock Solution | 100.0 | ml | - | - | |
| Fetal bovine serum* | 100.0 | g/l | - | - | |
| Distilled water | 800.0 | ml | - | - | |
| 1 OTEB-medium is available from Anaerobe Systems (http://www.anaerobesystems.com) | |||||
| 2 The following text was taken from the above page: - ORAL TREPONEME ENRICHMENT BROTH (OTEB) - Products: AS-603 Oral Treponeme Enrichment Broth (OTEB) 10 tubes / pkg | |||||
| 3 Intended Use: OTEB is an enriched medium for the isolation, cultivation and maintenance of Treponemes found as part of the oral flora. | |||||
| 4 OTEB is a complex peptone-yeast extract medium containing volatile fatty acids and serum for a source of long chain fatty acids. Volatile fatty acids and serum are necessary nutrients for recovery of Treponemes from the oral flora. Inoculation of the medium can be done using a gassing cannula or inside an anaerobic chamber. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. | |||||
| 5 Final pH 7.0 +/- 0.2 at 25 degrees C. | |||||
| 6 Final volume 7.0 ml +/- 0.7. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Glucose | 8 | g | 8 | 44.405 |
| Fructose | 8 | g | 8 | 44.405 |
| Sucrose | 8 | g | 8 | 23.371 |
| Maltose | 8 | g | 8 | 23.371 |
| Maltose | 8 | g | 8 | 23.371 |
| Ribose | 8 | g | 8 | 53.287 |
| Xylose | 8 | g | 8 | 53.287 |
| Pectin | 8 | g | 8 | 41.207 |
|
Starch
(soluble) |
8 | g | 8 | - |
| Sodium pyruvate | 8 | g | 8 | 72.701 |
| Thiamine pyrophosphate | 750 | µg | 7.5e-4 | 0.002 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 31.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 7.2 |
| Edit in Medium builder | |
Gas composition
- 85 % N2
- 10 % H2
- 5 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Fetal bovine serum | 100 | - |
| Sodium Chloride | 5 | 85.558 |
| Proteose peptone no. 2 | 5 | - |
| Yeast extract | 5 | - |
| Heart Infusion Broth | 5 | - |
| Show more | ||
