1381
1381 .
½ S° HELIORESTIS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thiocapsa imhoffii DSM 21303
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
anaerobic
28 °C
Main sol. 1381 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
EDTA
(1%) |
1.000 | ml | 0.01 | 0.034 | |
| KH2PO4 | 0.500 | g | 0.5 | 3.674 | |
| NH4Cl | 0.500 | g | 0.5 | 9.348 | |
| Bicine | 1.630 | g | 1.63 | 9.99 | |
| MgSO4 x 7 H2O | 0.200 | g | 0.2 | 0.811 | |
| CaCl2 | 0.075 | g | 0.075 | 0.676 | |
| Na2CO3 | 2.500 | g | 2.5 | 23.588 | |
| NaHCO3 | 2.500 | g | 2.5 | 29.759 | |
| Na-acetate | 1.000 | g | 1 | 12.19 | |
| Yeast extract | 0.100 | g | 0.1 | - | |
| Na2S x 9 H2O | 0.250 | g | 0.5 | 2.082 | |
| Na-pyruvate | 1.000 | g | 1 | 9.087 | |
| Trace elements | 1.000 | ml | - | - | |
| Vitamin B12 | 20.000 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Prepare under N2 gas and add vitamin B12, NaHCO3, Na2CO3 and sulfide as single sterile stock solutions to sterile medium after autoclaving. Adjust pH to 9 - 9.5. | |||||
Solution occurs in 2
other media (first defined in Medium 1147).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| EDTA | 5.2 | g | 5.2 | 17.794 |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| MnCl2 x 4 H2O | 0.1 | g | 0.1 | 0.505 |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 |
| Na2MoO4 x 2 H2O | 188.0 | mg | 0.188 | 0.777 |
| NiCl2 x 6 H2O | 25.0 | mg | 0.025 | 0.193 |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 |
| VOSO4 x 2 H2O | 30.0 | mg | 0.03 | 0.151 |
| Na2WO4 x 2 H2O | 2.0 | mg | 0.002 | 0.006 |
| NaHSeO3 | 2.0 | mg | 0.002 | - |
| Deionized water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 14.02.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 - 9.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Thiocapsa imhoffii DSM 21303
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 2.5 | 29.759 |
| Na2CO3 | 2.5 | 23.588 |
| Bicine | 1.63 | 9.99 |
| Na-acetate | 1 | 12.19 |
| Na-pyruvate | 1 | 9.087 |
| Show more | ||
