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DSMZ

DSMZ 1398 . 1/10 PYGV MEDIUM modified

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1398 #

ml
Equipment needed: Autoclave Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
S I 75.0 ml - -
S II 75.0 ml - -
Trypticase 1.0 g 1 -
Yeast extract 0.5 mg 5.0e-4 -
Glucose 1.0 g 1 5.551
L-Cysteine HCl 0.3 mg 3.0e-4 0.002
Resazurin
(0.1%) 		0.5 ml 5.0e-4 0.002
100x Vitamin solution 10.0 ml - -
Distilled water 1000.0 ml - -
1 SII
2 pH 7.0
3 Prepare the medium without the vitamin solution under an atmosphere of N2 and dispense into Hungate tubes. Seal under an atmosphere of nitrogen with rubber stoppers and autoclave. Aseptically add the vitamin solution from a filter sterilised stock solution to the sterile medium.

100x Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.1 g 0.1 0.409
Cobalamine 0.1 g 0.1 -
Thiamine-HCl x 2 H2O 0.5 g 0.5 1.339
p-Aminobenzoic acid 0.3 g 0.3 2.188
Pyridoxine 1.5 g 1.5 8.866
Riboflavin 0.5 g 0.5 1.328
Folic acid 0.5 g 0.5 1.133
Distilled water 1000.0 ml - -

S I #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 6 g 6 34.448
Distilled water 1000 ml - -

S II #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 6.0 g 6 44.09
(NH4)2SO4 12.0 g 12 90.813
NaCl 12.0 g 12 205.339
MgSO4 x 7 H2O 1.2 g 1.2 4.869
CaCl2 x 2 H2O 1.2 g 1.2 8.162
Distilled water 1000.0 ml - -

Metadata

Last modified: 31.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 1 5.551
Trypticase 1 -
NaCl 0.9 15.4
(NH4)2SO4 0.9 6.811
KH2PO4 0.45 3.307
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen obligate anaerobe 1 strain
temperature mesophilic 1 strain