DSMZ 142 . THIOMICROSPIRA PELOPHILA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 25.00 g 24.876 425.661
(NH4)2SO4 1.00 g 0.995 7.53
MgSO4 x 7 H2O 1.50 g 1.493 6.056
CaCl2 x 2 H2O 0.42 g 0.418 2.843
Trace element solution (Vishniac and Santer, 1957) 0.20 ml - -
Bromothymol blue
(0.1% w/v)
4.00 ml 0.004 0.006
K2HPO4 0.50 g 0.498 2.856
Na2S2O3 x 5 H2O 5.00 g 4.975 20.046
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except hydrogenphosphate, thiosulfate and vitamins), adjust pH to 7.2 and autoclave. K2HPO4 and Na2S2O3 are autoclaved separately each in 10% of the final volume. Filter sterilize the vitamins solution. Adjust pH of the complete medium to 7.2 with sterile 0.4% (w/v) Na2CO3 solution. Acidification of the medium during growth causes the pH indicator bromothymol blue to turn from blue to yellow.
2 Note: Growth of most Thiomicrospira strains is more reliable if the medium is prepared under a 80% N2 and 20% CO2 gas atmosphere to make it anoxic and then filled under air atmosphere into Hungate-type tubes (5 ml per vial). The pH is adjusted with a sterile stock solution of Na2CO3 (5% w/v) after autoclaving.
Solution occurs in 4 other media (first defined in Medium 69).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 50.00 g 50 148.718
ZnSO4 x 7 H2O 22.00 g 22 76.506
CaCl2 x 2 H2O 5.54 g 5.54 37.683
MnCl2 x 4 H2O 5.06 g 5.06 25.568
FeSO4 x 7 H2O 5.00 g 5 17.985
(NH4)6Mo7O24 x 4 H2O 1.10 g 1.1 0.89
CuSO4 x 5 H2O 1.57 g 1.57 6.288
CoCl2 x 6 H2O 1.61 g 1.61 12.4
Distilled water 1000.00 ml - -
1 Dissolve EDTA in distilled water, adjust pH to 7 using 2 N KOH, then add remaining compounds. Adjust pH of final solution to 6.0 with KOH.
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 05.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 75 % N2
  • 15 % CO2
  • 10 % O2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 18
Strains with modifications: 9

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.876 425.661
Na2S2O3 x 5 H2O 4.975 20.046
MgSO4 x 7 H2O 1.493 6.056
(NH4)2SO4 0.995 7.53
K2HPO4 0.498 2.856
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Cultivation metadata from 18 strains BacDive

pH 6 - 8 3 strains
pH 4 - 6 3 strains
pH 8 - 10 2 strains
phyla 1 18 strains
nutrition chemolithoautotroph 3 strains
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