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1474. PROLIXIBACTER MEDIUM GYSW

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.54 g 0.529 9.887
2 0.14 g 0.137 1.008
3 0.20 g 0.196 0.964
4 0.15 g 0.147 0.999
5 Trace element solution 10.00 ml - -
6 Vitamin solution 10.00 ml - -
7 Resazurin solution 1.00 ml - -
8 1.80 g 1.763 9.786
9
(Difco)
1.00 g 0.979 -
10 2.52 g 2.468 29.381
11 0.50 g 0.49 2.788
12 0.50 g 0.49 2.039
13 1000.00 ml 977.676 54269.2
Instructions:
  1. Prepare the liquid medium without Vitamin solution, Cysteine- HCl x H2O and Na2S x 9 H2O. Adjust to pH 7.0 with 1 M NaOH. Dispense the medium into Hungate tubes or serum bottles under gassing with N2:CO2 (80:20 v/v). Sterilize by autoclaving. Before inoculation add from sterile stock solutions Cysteine- HCl x H2O, Vitamin solution (filter sterilized) and Na2S x 9 H2O.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.800 g 12.8 66.967
2 1.350 g 1.35 4.995
3 0.100 g 0.1 0.592
4 0.024 g 0.024 0.101
5 0.100 g 0.1 0.68
6 0.100 g 0.1 0.734
7 0.025 g 0.025 0.111
8 0.010 g 0.01 0.162
9 0.024 g 0.024 0.099
10 1.000 g 1 17.111
11 0.120 g 0.12 0.505
12 0.004 g 0.004 0.015
13 0.004 g 0.004 0.014
14 1000.000 ml 998.207 55408.8
15 , required for dissolving NTA x - -
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
  2. Final pH 7.0 (with KOH).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 mg 1.0e-3 0.004
2 1000 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Filter, Hungate tubes

Associated strains

All strains for this medium: 3

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaOH 39.997 1000
NaHCO3 2.468 29.381
D-Glucose 1.763 9.786
Yeast extract 0.979 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains

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