Loading ...

DSMZ 1629 . MINIMAL MEDIA CONTAINING METHANOL

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.62 g 1.62 30.286
MgSO4 x 7 H2O 0.20 g 0.2 0.811
K2HPO4 2.40 g 2.4 13.779
NaH2PO4 x 2 H2O 1.10 g 1.1 9.168
Na2-EDTA 15.00 mg 0.015 0.045
FeSO4 x 6 H2O 3.00 mg 0.003 0.02
ZnSO4 x 7 H2O 4.50 mg 0.005 0.016
CoCl2 x 6 H2O 3.00 mg 0.003 0.023
MnCl2 x 4 H2O 0.64 mg 6.4e-4 0.003
H3BO3 1.00 mg 1.0e-3 0.016
Na2MoO4 x 2 H2O 0.40 mg 4.0e-4 0.002
CuSO4 x 2 H2O 0.30 mg 3.0e-4 0.002
CaCl2 x 6 H2O 3.00 mg 0.003 0.014
Methanol 5.00 ml 3.96 123.588
Agar 15.00 g 15 44.599
Distilled water 1000.00 ml - -
1 Prepare the medium without methanol. Adjust pH to 7.1.
2 Autoclave and after cooling to 50°C add the methanol from a filter-sterilized stock solution.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 12

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Methanol 3.96 123.588
K2HPO4 2.4 13.779
NH4Cl 1.62 30.286
NaH2PO4 x 2 H2O 1.1 9.168
Show more

Cultivation metadata from 12 strains BacDive

phyla 1 12 strains
temperature mesophilic 12 strains

Latest comments

No comments for this medium.