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194. DESULFOBULBUS SP. MEDIUM (FRESHWATER)

Culture medium recipe

Vol.
×
Solution occurs in 1 other media (first defined in medium 194 )
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 940 ml - -
2 Solution B 1 ml - -
3 Solution C 30 ml - -
4 Solution D 10 ml - -
5 Solution E 10 ml - -
6 Solution F 10 ml - -
7 , may stimulate growth of some strains
(5% w/v)
20 mg/l 0.02 -
Instructions:
  1. Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10%(v/v) inoculum.
Solution occurs in 1 other media (first defined in medium 194 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3.00 g 3.185 22.421
2 0.20 g 0.212 1.56
3 0.30 g 0.318 5.954
4 1.00 g 1.062 18.164
5 0.40 g 0.425 2.089
6 0.50 g 0.531 7.12
7 0.15 g 0.159 1.083
8 Selenite-tungstate solution 1.00 ml - -
9
(0.1% w/v)
0.50 ml 5.3e-4 0.002
10 940.00 ml 996.088 55291.2
Solution occurs in 1 other media (first defined in medium 194 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
Solution occurs in 1 other media (first defined in medium 194 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
Solution occurs in 1 other media (first defined in medium 194 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 150 1561.52
2 10.00 ml 998.207 55408.8
4 , might need strain adjustment 0.30 g/l - -
5 , might need strain adjustment 0.15 g/l - -
6 , might need strain adjustment 0.70 g/l - -
7 , might need strain adjustment 0.90 g/l - -
8 , might need strain adjustment 0.90 g/l - -
9 , might need strain adjustment 1.50 g/l - -
10 , might need strain adjustment 3.00 g/l - -
11 , might need strain adjustment 1.50 g/l - -
12 , might need strain adjustment 2.50 g/l - -
13 , might need strain adjustment 0.40 g/l - -
14 Seven vitamins solution , might need strain adjustment 1.00 ml/L - -
15 , might need strain adjustment 2.50 g/l - -
16 , might need strain adjustment 0.10 ml/l - -
18 , might need strain adjustment 2.20 g/l - -
19 , might need strain adjustment
(neutralized with NaOH)
0.60 g/l - -
21 , might need strain adjustment 1.00 g/l - -
22 , might need strain adjustment 1.50 g/l - -
Solution occurs in 1 other media (first defined in medium 194 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
Solution occurs in 1 other media (first defined in medium 194 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.4 g 40 166.54
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 36
Strains with modifications: 36

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.202 55353.1
Na2SO4 2.991 21.055
Na-propionate 1.499 15.6
Na2CO3 1.499 14.138
NaCl 0.997 17.058
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Cultivation metadata from 36 strains BacDive

halophily not 2 strains
halophily slightly 1 strain
halophily moderate 1 strain
pH 6 - 8 3 strains
pH 8 - 10 2 strains
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