207
207 .
PROPIONISPIRA MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 207 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.3 | g | 0.3 | 2.205 | |
| Na2HPO4 x 12 H2O | 2.1 | g | 2.1 | 14.793 | |
| NH4Cl | 1.0 | g | 1 | 18.695 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| Trace element solution | 10.0 | ml | - | - | |
| FeSO4 x 7 H2O | 1.5 | mg | 0.002 | 0.005 | |
| Wolin's vitamin solution | 5.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| NaHCO3 | 4.0 | g | 4 | 47.615 | |
| Yeast extract | 2.0 | g | 2 | - | |
| Na-lactate | 5.0 | g | 5 | 44.619 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.2. Gas atmosphere: 80% N2 + 20% CO2. Prepare a 5% (w/v) solution of sodium sulfide anoxically under nitrogen and autoclave separately. Before use, neutralize by dropwise addition of 1 N HCl. | |||||
Solution occurs in 8
other media (first defined in Medium 144).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
12.80 | g | 12.8 | 66.967 | |
| FeCl2 x 4 H2O | 0.20 | g | 0.2 | 1.006 | |
| MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
| CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnCl2 | 0.10 | g | 0.1 | 0.734 | |
| CuCl2 | 0.02 | g | 0.02 | 0.149 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.03 | g | 0.03 | 0.231 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| Na2SeO3 x 5 H2O | 0.03 | g | 0.03 | 0.114 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml. | |||||
Solution occurs in 53
other media (first defined in Medium 141).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| (DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na-lactate | 5 | 44.619 |
| NaHCO3 | 4 | 47.615 |
| Na2HPO4 x 12 H2O | 2.1 | 14.793 |
| Yeast extract | 2 | - |
| NH4Cl | 1 | 18.695 |
| Show more | ||
