DSMZ 207 . PROPIONISPIRA MEDIUM

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Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.3 2.205
Na2HPO4 x 12 H2O 2.1 g 2.1 14.793
NH4Cl 1.0 g 1 18.695
MgCl2 x 6 H2O 0.2 g 0.2 0.984
Trace element solution 10.0 ml - -
FeSO4 x 7 H2O 1.5 mg 0.002 0.005
Wolin's vitamin solution 5.0 ml - -
Resazurin 1.0 mg 1.0e-3 0.004
NaHCO3 4.0 g 4 47.615
Yeast extract 2.0 g 2 -
Na-lactate 5.0 g 5 44.619
Na2S x 9 H2O 0.5 g 0.5 2.082
Distilled water 1000.0 ml - -
1 Adjust pH to 7.2. Gas atmosphere: 80% N2 + 20% CO2. Prepare a 5% (w/v) solution of sodium sulfide anoxically under nitrogen and autoclave separately. Before use, neutralize by dropwise addition of 1 N HCl.
Solution occurs in 8 other media (first defined in Medium 144).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
12.80 g 12.8 66.967
FeCl2 x 4 H2O 0.20 g 0.2 1.006
MnCl2 x 4 H2O 0.10 g 0.1 0.505
CoCl2 x 6 H2O 0.17 g 0.17 1.309
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnCl2 0.10 g 0.1 0.734
CuCl2 0.02 g 0.02 0.149
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
NaCl 1.00 g 1 17.112
Na2SeO3 x 5 H2O 0.03 g 0.03 0.114
Distilled water 1000.00 ml - -
1 First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na-lactate 5 44.619
NaHCO3 4 47.615
Na2HPO4 x 12 H2O 2.1 14.793
Yeast extract 2 -
NH4Cl 1 18.695
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain