246
246 .
SEA WATER AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Vibrio owensii DSM 2165
* Modification: Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 514. Subsequent subculturing may be carried out in liquid medium or on agar plates of media 514 or 246.
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Main sol. 246 #
ml
Solution occurs in 1
other media (first defined in Medium 246).
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Beef extract | 10 | g | 10 | - | |
| Peptone | 10 | g | 10 | - | |
| Agar | 20 | g | 20 | 59.465 | |
| Tap water | 250 | ml | - | - | |
| Sea water | 750 | ml | - | - | |
| 1 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Solution occurs in 5
other media (first defined in Medium 243).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 28.13 | g | 28.13 | 481.348 |
| KCl | 0.77 | g | 0.77 | 10.329 |
| CaCl2 x 2 H2O | 1.60 | g | 1.6 | 10.883 |
| MgCl2 x 6 H2O | 4.80 | g | 4.8 | 23.61 |
| NaHCO3 | 0.11 | g | 0.11 | 1.309 |
| MgSO4 x 7 H2O | 3.50 | g | 3.5 | 14.2 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 20 | 59.465 |
| Peptone | 10 | - |
| Beef extract | 10 | - |
| Tap water | - | - |
| Sea water | - | - |
