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38. ACETOANAEROBIUM MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 20 -
2 10.00 g 10 -
3 1.04 g 1.04 5.971
4 0.68 g 0.68 4.997
5
(0.1% w/v)
0.50 ml 5.0e-4 0.002
6 0.15 g 0.15 0.625
7 1000.00 ml 998.207 55408.8
Instructions:
  1. Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Light

1 strain needs light for growth. Select a strain for more details.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Tryptone 20 -
Yeast extract 10 -
K2HPO4 1.04 5.971
KH2PO4 0.68 4.997
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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