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DSMZ

DSMZ 465c . MINERAL MEDIUM WITH DICHLOROMETHANE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Methylophilus leisingeri DSM 6813

* Modification: For DSM 6813, add 10 ml/l instead of 1 ml/l trace element solution SL-4.

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 2-3 days aerobic 30 °C

Main sol. 465c #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 465 1000 ml - -
Trace element solution SL-4* 10 ml - -
1 Prepare medium 465 (addition of 50 mg/l bromothymol blue as pH-indicator is recommended) and autoclave. Grow the cells in medium 465 with methanol supplied via incubation atmosphere (up to 10 ml methanol per liter medium). Incubate these adapted cells in medium 465 and consecutively feed small amounts of dichloromethane (toxic! 2 mmol per liter or less) via incubation atmosphere. Readjust pH of the culture with sterile 1 M NaOH if necessary.

Medium 465 #

1000
Solution occurs in 7 other media (first defined in Medium 465).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.50 g - -
KH2PO4 1.00 g - -
(NH4)2SO4 0.50 g - -
MgCl2 x 6 H2O 0.10 g - -
Ca(NO3)2 x 4 H2O 0.05 g - -
Trace element solution SL-4* 1.00 ml - -
Distilled water 1000.00 ml - -
1 pH 7.25
2 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2
Strains with modifications: 1
Methylophilus leisingeri DSM 6813

+  10 ml Trace element solution SL-4
-  Trace element solution SL-4

Media similar to medium 465c

465f MINERAL MEDIUM WITH 3-AMINOPHENOL
465i MINERAL MEDIUM WITH ATRAZIN

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 24.655
KH2PO4 1 7.348
(NH4)2SO4 0.5 3.784
MgCl2 x 6 H2O 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 0.212
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains